Welcome to KBDL
The goal of the KBDL is provide computational support
and resources to users of the University of Louisville Molecular Neurobiology Core
Facility. It has been designed as a state-wide resource for researchers in multiple
institutions, especially for those who are users of our KBRIN gene-microarray facility.
First, the KBDL consists of a web-accessible SQL Server database for the storage of
datasets associated with gene-array data and other associated assays. Currently,
this version allows Principal Investigators (PIs) and their co-workers to view their "raw" and “processed”
array data. The browser provides fold changes for each gene with a significance
level (T-test) value. The data is presented in a window which
can be scrolled to look for genes of particular interest.
Alternatively the PI can download an Excel spreadsheet. The KBDL is userid and password protected, and the data is backed up on a regular basis.
What can
you do with KBDL?
- You can view your data.
- You can search your data for particular genes.
- You can view images for particular genes in your
data (images from well known image databases).
- You can pin point up regulated or down regulated
genes in your T test files.
- You can cluster your data file through KBDL clustering
Tool.
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Second, the KBDL has a set of online data-mining tools to allow for a search for one or more genes in one or multiple files. The KBDL also provides a place to store associated data from other assays. For example, Real-Time PCR is used by our laboratory to confirm, for a subset of genes, those which are up-regulated, unchanged and down-regulated. Other validations may include protein screens such as immunoblotting and/or immunocytochemistry.
Third, the KBDL is an ongoing research and development
tool for the design team and collaborating researchers. The goal is to develop tools
which will allow more complete analyses of data as well as tools to aid data integration. Where
possible, we will include suitable links to additional useful sites including NCBI.
For example, for users who
work with mouse we include links from the processed data to GENSAT,Allen Brain
Atlas for
visualization of in situ hybridization data to the relevant genes.
The KBDL Design Team: Maha Soliman, Nigel Cooper
Supported by NIH: NCRR P20 RR16481